Interaction between beta-fluoro-oxaloacetate and glutamate-aspartate transaminase of heart mitochondria.
نویسندگان
چکیده
منابع مشابه
Interaction between p-Fluoro-oxaloacetate and Glutamate- Aspartate Transaminase of Heart Mitochondria”
In a previous publication (1) we have described the synthesis and chemical properties of /3-fluoro-oxaloacetic acid. This acid was found to be a potent inhibitor of mitochondriil malic dehydrogenase (1,2). It is probable that fluoro-oxaloacetate inhibits other enzymes which react with oxaloacetate. We have performed further experiments in order to test this hypothesis. As a part of this study t...
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The isolated aspartate/glutamate carrier and oxoglutarate carrier from mitochondria were coreconstituted into phospholipid vesicles. Reconstitution of the functionally active carrier proteins with high protein/lipid ratios was achieved by detergent removal on hydrophobic ion-exchange columns. A simplified version of the mitochondrial malate/aspartate shuttle was constructed by inclusion of glut...
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During glycine oxidation by spinach leaf mitochondria, oxygen consumption showed a strong and transient inhibition upon addition of oxaloacetate or aspartate plus alpha-ketoglutarate. During the course of the inhibition, aspartate and alpha-ketoglutarate were stoichiometrically transformed into malate and glutamate.It is concluded that oxaloacetate formed by transamination is reduced by the mal...
متن کاملComparative studies on glutamate metabolism in synpatic and non-synaptic rat brain mitochondria.
1. The apparent Michaelis constants of the glutamate dehydrogenase (EC 1.4.1.3), the glutamate-oxaloacetate transaminase (EC 2.6.1.1) and the glutaminase (EC 3.5.1.2) of rat brain mitochondria derived from non-synaptic (M) and synaptic (SM2) sources were studied. 2. The kinetics of oxygen uptake of both populations of mitochondria in the presence of a fixed concentration of malate and various c...
متن کاملGlutamate oxidation in liver mitochondria of rats fed on pyridoxine-deficient diet.
Two pathways are known to exist for glutamate oxidation in liver mito chondria. One is by way of glutamate dehydrogenase [EC 1.4.1.3.] reaction and the other by aspartate transaminase [EC 2.6.1.1.] reaction. Although both enzyme activities are high in mitochondrial fraction, it is known that glutamate is pre ferentially oxidized through the transaminase pathway in isolated intact mito chondria ...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 235 شماره
صفحات -
تاریخ انتشار 1960